Fig 1: Verification of BTN2/3 subfamily expression profiles and immune infiltration. (A) The differential expression clusters of PCGs in the glioma specimens and paracancerous tissues are shown. The suffix E-Signal represents edge tissues, the suffix T-Signal represents tumor tissues, and the suffix N-Signal represents normal tissues. The heatmap indicates the upregulated and downregulated PCGs. The expression levels of BTN2/3 subfamilies in the different tissues are displayed below. (B) The differential expression levels of BTN2/3 in the normal human astrocyte (NHA) cell line and GBM cell lines (U87 and U251). The results are presented as the mean ± SD from three independent experiments. (C) Results of immunohistochemical fluorescence staining. According to the qRT-PCR results of BTN2A2 and BTN3A, the glioblastoma clinical samples were divided into high-/low-expression groups, and immunohistochemical fluorescence staining was performed. Green signal, CD68, a panmacrophage biomarker; red signal, BTN2A2 or BTN3A, as presented in the figure; blue signal, DAPI. PCGs, protein-coding genes. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, no significance.